Effect of NMDA receptor antagonist on hepatic oxidative stress and inflammatory response induced by magnesium deficiency in rats

Abstract

Excessive action of N-methyl-d-aspartate (NMDA) receptor is induced by magnesium deficiency, which causes oxidative stress and inflammation in the heart. In the present study, we examined the effect of an NMDA receptor antagonist (dizocilpine, MK-801) on the hepatic oxidative stress and inflammatory response induced by magnesium deficiency in rats. Male Sprague-Dawley rats were divided into four groups of six rats as a 2 × 2 factorial arrangement (control and magnesium deficiency, and MK-801 and placebo treatment). The experiment was conducted for one week. Hepatic level of reactive oxygen species was increased by magnesium deficiency but MK-801 administration did not affect the level of reactive oxygen species. Magnesium deficiency increased the hepatic mRNA levels of Mac-1 and CD45 expressed by leukocyte-related cells such as macrophage, monocyte and neutrophil, and the mRNA level of Mcpt1 expressed by mast cells but MK-801 administration did not affect the increased levels of these mRNA by magnesium deficiency. As a conclusion, the excessive action of NMDA receptor was unlikely to be involved in hepatic oxidative stress and inflammatory response induced by magnesium deficiency in rats