2021 年 38 巻 p. 36-39
Biotin in foods is generally measured by a microbiological method using Lactobacillus plantarum (ATCC 8014). This bacterium responds not only to biotin but also to biotin d- and l-sulfoxides, which have no effect as biotin in rats. Since these analogs influence measured values, biotin and its analogs in foods were fractionated by column chromatography. Then, the proportion of growth activity of L. plantarum responding to these biotin compounds was examined. The results show that in mushrooms, the proportion of a compound presumed to be biotin d-, l-sulfoxides, or both was relatively high compared with egg yolk, chicken liver, and broccoli. This indicates that the biotin content in mushrooms may be overestimated when determined by L. plantarum.