中等度亜鉛欠乏がラットの肝臓および脾臓中非ヘム鉄濃度に与える影響

抄録

Iron biological parameters (hemoglobin concentration, hematocrit and serum iron concentration) are being used to check for iron nutritional condition. However, these parameters are changed by iron deficiency and other mineral deficiency, especially by zinc deficiency. We measured liver and spleen non-heme iron concentrations (supernatant and coagulum) in moderate zinc deficiency or with diet restriction. Thirty 3-week-old male Sprague-Dawley rats were randomly and equally divided to control group (Control), pair-fed group (PF) and moderately zinc-deficient group (ZD), and fed for 4 weeks. Non-heme iron fractions (supernatant and coagulum) were separated and measured by atomic absorption spectrometry. Differences between groups were statistically analyzed by the Brown-Forsythe ANOVA and post hoc Welch’s t-test. P value less than 0.05 was considered significant. Liver supernatant non-heme iron concen- tration was significantly increased by zinc deficiency and significantly decreased by diet restriction. Spleen superna- tant non-heme iron concentration was not significantly changed by ANOVA. Liver coagulum and spleen coagulum non-heme iron concentration were not significantly changed in all groups. These results suggest that iron accumula- tion in liver is remarkable by zinc deficiency per se, removing the effect of low food intake by paired-feeding technique.