To confirm whether flaxseed protein really has an effect on lipid metabolism, we extracted protein from flaxseed meal and administered it to mice. A sodium hydroxide solution adjusted to pH 7.5-9.0 was added to flaxseed meal (protein content 29.4%),0.1 M hydrochloric acid was added to the extract to adjust the pH to 3-4, and the resulting precipitate was washed and dried to obtain flaxseed protein with 85% protein content. One group (control group) was fed AIN93G diet in which casein was the protein source, and the other group (flaxseed protein group) was fed a diet in which linseed protein replaced half of the casein in the AIN93G diet. Lysine, the limiting amino acid of flaxseed protein, was added to the diet of the flaxseed protein group in the required amount. Body weight at the end of the feeding period was slightly lower in the flaxseed protein group than in the control group, but this difference was not significant. There were no differences between groups in the concentrations of serum proteins and lipid-related components (triglycerides, total cholesterol, and total lipids). There were also no clear differences in liver triglycerides and cholesterol concentrations between the two groups. These results indicate that flaxseed protein is unlikely to have lipid metabolism-improving effects.