S24 PROTEIN ENGINEERING IN STUDIES ON METABOLISH OF TOXIC SUBSTANCES

Abstract

Expression of cytochrome P-450s, drug metabolizing enzymes, from their coding sequences in heterologous cells were developed and the enzymatically active P-450s were synthesized. cDNAs of various chimera or mutant P-450s were constructed and expressed. Thus, regions or residues important for the function of cytochrome P-450 were analyzed.