2009 Volume 62 Issue 9 Pages 709-712
A four-day-old Japanese Black Cattle calf presented with severe watery diarrhea, which lead to depression and bloody stool, followed by death, in Ishigaki Island, Japan on June 2007. Vibrio cholerae was isolated from the brain, heart, lungs and major organs in a pure culture. The strains were identified as serogroup O135. Immunohistochemical examination revealed a large number of V. choleraeo O135 antigens in the brain, lungs and thymus. The isolates do not produce cholera enterotoxin, while harbor hlyA and toxR genes. An epidemiological investigation showed that V. cholerae O135 was not isolated in environmental samples collected from the farm, whereas V. cholerae O14, O19, O27 and O170 were isolated from the seawater samples around coastal areas in remote islands respectively.
