Development of BPV-Genotyping by PCR-RFLP and a Practical Example of that Method

Abstract

A practical polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify bovine papillomavirus (BPV) genotypes was developed and was compared to the conventional method (CM). Bovine papillomas were collected from dairy heifers in a grazing facility in Shizuoka. The CM detected BPV9 (19/92), BPV6 (56/92) and BPV10 (7/92) and all positive samples could be identified by PCR-RFLP. These results suggested that PCR-RFLP is a useful method for identifying BPV genotypes.