1969 Volume 22 Issue 12 Pages 600-605
When inoculated intramuscularly with 1.0 ml (104.5EID50per capita) of the virulent strain of Newcastle disease virus, pigeons manifested nervous symptoms, including locomotive paralysis as the principal sign, for 26 days. There was almost parallel relationship between general symptoms and the phase of propagation of virus in the body. The antibody titer rose markedly after virus propagation had reached a peak. Nervous symptoms were apparent at this time. The virus was demonstrated over a period of 3 to 10 days after inoculation, the peak falling at 5 days. Its titer was 106.25EID50/0.1ml in the lung, 105.5in the brain, 105.0in the liver, 104.67 in the spleen, 104.5in the trachea, 102.5in the intestinal contents, and 102.25in the blood. A virus transmission experiment was carried out on chickens with the feces of infected pigeons. When such feces contained virus, the incculated chickens died after showing clinical symptoms, and the virus was recovered from them.