An Improved Method for Making Blood Films and

Results from Bovine Blood

Abstract

A simple method was devised for making blood films. It was performed in the following manner.
The end without chemical of a commercial matchstick was dipped, about 5mm in depth in blood contained in a tube with the anticoagulant EDTA. Then, by the aid of the stick, 5 lines of blood smear were made on a slide in parallel with the long side of the slide. At the time of smearpreparation, it was not necessary to take the moving speed of the stick, and the angle between the slide and the stick into consideration.
Blood films prepared by this method had always portions of any line on the slide suitable for microscopic observation on red and white blood cells and thrombocytes. Besides, there was no significant difference between the conventional silde method and this method in the rate of appearance of any of the 5 kinds of leukocytes.