Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
A Counting Method of Coccidial Oocysts with Chrome-Sulfuric Acid
Toshio ISHIIH. OHNAGA
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1970 Volume 23 Issue 3 Pages 133-137

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Abstract

Chrome-sulfuric acid was added to a fecal sample to decompose and dissolve the fecal contents, so that the counting of coccidial oocysts might be made with ease.
1. The chemical used was a mixture of equal volumes of 20 % sodium bichromate and concentrated sulfuric acid.
2. It was desirable to finish the oocyst counting within 30 minutes after the chemical treatment in order to prevent the oocysts from being disintegrated.
3. A fecal sample unfiltrated and containing a large amount of residual feed sometimes became foamy. This disadvantage could be prevented by increasing the amount of chrome-sulfuric acid to be added.

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