Isolation of Bovine Rhinovirus and Parainfluenza Virus Type 3 from Cattle Showing Respiratory Symptoms and an Antibody Survey on Cattle

Abstract

Seven cytopathogenic viruses and eleven bovine hemagglutination viruses were isolated from cattle in six outbreaks of respiratory disease in Hiroshima Prefecture over a period from 1974 to 1977. The former were neutralized with antiserum against the M-17 strain of bovine rhinovirus type 1. One (50-248-1 strain) of them was readily inactivated by acid, trypsin, and heating at 50°C for 60 minutes, but resisted ether and chloroform. The type of nucleic acid of the virus was determined to be RNA.
The hemagglutinating properties of ten of the eleven bovine hemagglutination viruses were inhibited by antiserum against parainfluenza virus type 3 (PI-3).
Cattle involved in the outbreaks showed fever, coughing, nasal secretion, and so on.
A significant rise in antibody titer was shown at the same time against bovine rhinovirus, PI-3, and bovine adenovirus type 7 (BAd-7) in two cattle involved in the six outbreaks. Antibody titers were shown against bovine rhinovirus, bovine respiratory syncytial virus (BRS), PI-3, and BAd-7 in two cases, and against bovine rhinovirus, BRS, and BAd-7 in two cases. An antibody titer against bovine rhinovirus was noticed in 86% of the serum samples collected from cattle in Hiroshima Prefecture.