Frozen Storage, Culture and Transfer of Bovine Embryos
1983 Volume 36 Issue 11 Pages 643-647
Details
1983 Volume 36 Issue 11 Pages 643-647
A total of 66 bovine embryos were frozen in glass ampoules (6 × 70mm) and stored in liquid nitrogen. Then, they were thawed rapidly in water at 37°C. The storage medium used was Dulbecco's PBS + 25% calf serum containing 1.0M glycerol. Embryos in the storage medium were cooled from 35°C to - 5°C at a rate of 1.0°C/min. and seeded at-5°C. After seeding they were cooled to -36°C at 0.3°C/min. or at 1.0°C/3 min. before being transferred directly to liquid nitrogen.
As a result, 54 embryos were cultured for 24-48 hours, 12 embryos transplanted to 12 recipients, and 15 embryos (27.8%) survived after culture. Three recipients (25.0%) were pregnant and gave birth to 3 normal calves.
