1983 Volume 36 Issue 2 Pages 68-73
Canine parvovirus (CPV) was isolated in primary canine kidney cell and feline kidney (FK) cell cultures. It was detected from intestine and feces from eleven of 21 dogs clinically and pathologically suspected of CPV infection from 1980 to 1981.
The isolated viruses were propagated with intranuclear inclusion bodies in primary FK cell and feline lung cell cultures and agglutinated porcine and feline erythrocytes.
The serological properties of the isolated viruses were identical with those of CPV and feline panleukopenia virus. The serotype of the isolated strains was proved to be of monotype by the hemagglutination-inhibition (HI) test.
In experimental infection with an isolate, clinical symptoms and pathologic lesions similar to those in field cases were induced. The virus was recovered from almost all the organs of dead pups.
A serologic survey was conducted on 748 street dogs in various districts of Japan over a period from 1977 to 1981. HI antibody-positive sera first appeared in October, 1978. Since 1979, seropositive dogs of high HI antibody have been increasing in number. The result demonstrated that CPV was wide spread among the dogs in Japan.
