Isolation and Early Fluorescent Antigen Detection of Bovine Respiratory Syncytial Virus in Vero Cell Culture
1985 Volume 38 Issue 7 Pages 436-442
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1985 Volume 38 Issue 7 Pages 436-442
An attempt was made to isolate bovine respiratory syncytial virus (BRSV) from nasal secretions of 18 effected cattle in two herds by using cultures of Vero cells (VC) and bovine kidney cells (BK). The virus was isolated from 11 cattle (61%) in VC and 3 cattle (3%) in BK. CPE was clearey in VC than in BK. Specific fluorescent cells against BRSV were observed in VC 24 hours after inoculation. They were noticed in 10 cattle (55%) 4 days after inoculation.
