1986 Volume 39 Issue 7 Pages 439-443
Pack culture was prepared as follows. An agar plate with microaerophilic bacteria was put in a polyethylene bag, from wihch the air was removed by an aspirator. Then, the bag was filled with 10% carbon dioxide gas and sealed with a polysealer. It was kept within the incubator. By this method 24 species of pathogenic bacteria have been isolated at the authors' laboratory since 1979.
Dry stock was performed by stamp's gelatin disc method modified in the following manner. Drops of a condensed bacterial suspension were instilled on a thin polyethlene film, instead of a paraffin film. When that film was dried in a desiccator, the disc was liberated easily. Bacteria could be stored for a long period when the disc was kept within a sealed small polyethylene beg at 5°C. A total of 351 strains of 31 bacterial species isolated at the authors' laboratory were stored by this method for 1 to 5 years.