Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Safety and Immunogenicity on Piglets of Porcine Parvovirus Attenuated by Serial Passages at Low Temperature
SEIKA MARUYAMAFUMIHIKO MARUYAMAMICHIO MORITA
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1987 Volume 40 Issue 5 Pages 360-364

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Abstract

Four piglets 5 months old were inoculated subcutaneously with 106.0 TCID50/ml of the HT--SK-C strain of porcine parvovirus (PPV) that had been adapted to primary swine kidney cell culture at low temperature.
None of them presented any abnormal clinical sighn, viremia or virus discharge. Four weeks after inoculation, 2 of them and a cohabiting control piglet were challenged orally with 106.0TCID50/ml of a field strain (90HS strain). One of the inoculated piglets exhibited weak viremia, but not leukopenia or virus discharge. The control piglet exhibited viremia, leukopenia and virus discharge. HI and neutralizing antibody titers rose rapidly in it after challenge.
HI antibody titer increased to 1: 160-1: 640 and neutralizing antibody titer to 1: 64-1: 256 2-3 weeks after inoculation. HI and neutralizing antibody titer were 1: 80 and 1: 16-1: 64, respectively, 12 weeks after inoculation.

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