1987 Volume 40 Issue 7 Pages 498-502
An ldentification of 142 isolates of staphylococci from normal cow's milk was made by using kloos and Schleifer's simplified scheme. Its result was compared with that of identification by the Api staph system. Antibiotic susceptibilities of 138 of these isolates were carried out routinely by the agar dilution method with 10 antibiotics.
The 142 isolates were identified as follows by the conventional tests: S. taphylococcus aureus, 17 (12.0%), S. simulans 49 (34.5%), S. xylosus, 30 (21.1%), S. saprophyticus, 13 (9.2%), S. cohnii, 12 (8.5%) and S. hominis, 10 (7.0%). The remaining 11 (7.7%) were not identified. The agreement of the result of identification by the Api staph systme was as follows: 100% for S. aureus, 89.8% for S. simulans and 60% for S. xylosus. As a whole, 55.6% of the isolates tested were identified correctly. All the isolates tested were susceptible to durgs, such as PC·G, AB·PC, CX, KM, CZ and S·T combination. Of the 138 isolates, 16 (11.6%), 8 (5.8%) and 1 (0.7%) were resistant to OTC and SM, CTC, and FM, respectively.