Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Evaluation of Multilayered Dry Chemistry System for Canine Blood Chemistry Test
YOSHIMASA SAKAMOTOYOSHIMICHI MIYAZAWAHIROFUMI ANDO
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1991 Volume 44 Issue 11 Pages 1121-1128

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Abstract

To evaluate the usefulness of a mutilayered dry chemistry system (KONICA DRYLABO SYSTEM, Chugai Pharmaceutical Co. Ltd.), total cholesterol (T-Cho), blood urea nitrogen (BUN), triglycerides (TG), total bilirubin (T-Bil), aspartate aminotransferase (AST or GOT), alanine aminotransferase (ALT or GPT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) in canine plasma treated with heparin or EDTA-2Na, were measured by the dry and routine wet (RaBA-Σ, ChugaiPharmaceutical Co. Ltd., and Hitachi Auto Analyzer, Hitachi Ltd.) chemistry systems. The correlation coefficients in each analyte between the dry and two wet chemistry systems ranged from 0.761 to 0.992. Slopes of 1.341 to 1.888 were found in T-Bil (RaBA), ALP (RaBA and Hitachi) and CPK (Hitachi). In LDH, the slope was 0.774 (RaBA) or 0.729 (Hitachi). The variation of coefficients in this dry chemistry system ranged from 1.5 to 11.3% in the intra-assay, and from 2.2 to 15.1% in the inter-assay except for CPK. Compared with serum or samples without hemolysis, the anticoagulants (heparin and EDTA-2Na) and hemolysis (Hb concentration: about 50-200mg/dl) significantly affected values measured by the dry chemistry system.
It appears that the operation of the analyzer is simpler, measuring time shorter and sample volume smaller (10μl) in this dry chemistry than in routine wet chemistry analyzer. However, the interpretations in case of the interferance Of anticoagulants or hemolysis should be taken into consideration in the dry chemistry system.

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