1991 Volume 44 Issue 12 Pages 1180-1184
An enzyme-linked immunosorbent assay (ELISA) for detecting antibody to glycoprotein OH of Aujeszky's disease virus (ADV) was evaluated in comparison with the serum neutralization (NT) and latex agglutination tests. A total of 1128 sera were collected from pigs of both ADV-free and infected farms. The sensitivity and specificity of the Off ELISA, relative to NT, were 99.7% and 99.9%, respectively. A judgement agreement between both tests was 99.8%. In pigs experimentally inoculated with five field isolates of ADV, the g III ELISA detected antibodies from post-inoculation day 7 to 14. Pigs vaccinated with g III-deleted vaccine were positive for antibodies by the NT but negative by the On ELISA, and produced anti-g III antibody after being exposed to a virulent ADV. The present results indicate that pigs infected with virulent ADV can be differentiated from those received g III-deleted vaccine by the g III ELISA, and a vaccination program using the g III-deleted vaccine, in conjunction with the g III ELISA, would contribute to the eradication of Aujeszky's disease.
