1992 Volume 45 Issue 3 Pages 170-173
A 10-month-old grazing Holtsein cow died after showing bloody diarrhea and dehydration in Niigata Prefecture. Necropsy revealed mucosal congestion with adherent mucous and blood clots, and severe serosal edema in the ileum and colon. Histologically, the ileum and colon had extensive congestion and hemorrhage in the lamina propria, and submucosal edema. Amphophilic intranuclear inclusion bodies were located in the swollen endothelial cells of the capillary and small vessels in the lamina propria and submucosa. The inclusion bodies were also detected in endothelial cells of the blood vessels in the mesenteric lymph nodes, kidneys and spleen. The inclusion bodies showed a positive reaction against rabbit anti-bovine adenovirus serotypes 1, 3, and 7 sera by the immunoperoxidase technique. Ultrastructurally, adonovirus-like particles were demonstrated in intranuclear inclusion bodies.