1993 Volume 46 Issue 2 Pages 117-119
Indirect fluorescent antibody (IFA) technique using a monoclonal antibody (M-Ab) specific to nucleocapsid protein of avian infectious bronchitis (IB) virus was applied to diagnosis of IB of chickens. Since affected chickens of 69 days of age showed respiratory signs and severe diarrhea, they were suspected of IB clinically. The IB virus specific antigens were clearly demonstrated in thin sections of the swollen kidneys collected from chickens. The IB virus was isolated from the kidneys and virus neutralizing antibody titers against the isolates rose significantly in convalescent sera of chickens. From these results, the disease was diagnosed as IB, and the IFA technique using M-Ab was judged to be usuful for diagnosis of IB.
