Detection of Benzimidazole Anthelmintics by High Performance Liquid Chromatography

Abstract

Recovery of 6 benzimidazole anthelmintics (BIZs) artificially added in pork muscle and liver was performed by high performance liquid chromatography (HPLC) using Shim-pack CLC-ODS. Samples were deprotenized and extracted with acetonitrile, followed by Sep-PaK C₁₈ and then Sep-PaK silica (liver) cleanup procedures, and they were subjected to HPLC with a mobile phase solution containing CH₃CN and 50mM NaH₂PO₄ (35: 65) and detection of BIZs by ultra violet (295nm). Recoveries of the drug from muscle and liver samples containing 1μg/g were 88 to 92% and 81 to 96%, respectively, and detection limit for each drug was 2 to 40ng/ml.