Protective Effect of Dithiothreitol on Chlorothalonil-Induced Cytotoxicity in Rat Hepatocyte Culture

Abstract

The primary culture of rat hepatocytes was exposed to chlorothalonil (TPN) for 24hr, and cytoxicity was evaluated by the 50% leakage of lactate dehydrogenase (LDH). TPN was potent toxic agent among 8 pesticides tested; the rank order of cytotoxicity was oxine-cupper>TPN>fenitrothion (MEP)>diazinon> chlorpyrifos>trichlorfon (DEP)>asulam=simazin (CAT). Cell injury with marked decrease in cellular glutathione level induced by TPN was inhibited by treating with dithiothreitol.