Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Identification and Quantitative Determination of Residual New Quinolone Antibacterial in Pork Meat by Bioassay and HPLC
Kiyoshi YASUFUKUMasayuki KAMEMOTOToshio IWASAKIIwao MORIKOUCHI
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JOURNALS FREE ACCESS

1998 Volume 51 Issue 2 Pages 100-104

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Abstract

A residual antibacterial was detected from slaughtered swine tissues by a simplified bioassay, and it was suspected of a new quinolone by a classified bioassay and identified as enrofioxacin (ERFX) by TLC-microbioautography. The residual ERFX was also identified by HPLC with fluorescence and photodiode-array detectors. The content of ERFX was 0.33μg/g of the cervical muscles by HPLC, and the detection limit was 0.001 μg/ml by fluorescence detector.

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