2004 Volume 57 Issue 12 Pages 785-788
To isolate the non-cytopathogenic bovine viral-diarrhea virus (BVDV) from serum specimens obtained from cattle persistently inftcted with it, we evaluated a new rapid assay using the bovine kidney-cell line (MDBK-SY) and a serum-free culture medium. Serum specimens (0.01ml) were inoculated with 0.19ml of MDBK-SY cell suspension and cultured for 8days. On the fifth day after inoculation, the cytopathic efftct (CPE) was observed in 19 serum specimens obtained ftom cattle persistently infected with the BVDV (102.5-105.1 TCID50/ml). Only 3 of 1, 510 field serum specimens induced the CPE. We therefore conclude that this assay is useful in identifying cattle infected with BVDV from a large number of specimens.