Journal of the Japan Veterinary Medical Association
Online ISSN : 2186-0211
Print ISSN : 0446-6454
ISSN-L : 0446-6454
Quick and Reliable Method for Diagnosis of Swine Erysipelas during Meat Inspection
Hideki FUJIMOTOTomoko SAKODARyutaro TANAKAIkumi GOHARAManabu YAMAGUCHITomoko YAMAMOTOSeiji TOYOSHIMATakeshi OBI
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2006 Volume 59 Issue 10 Pages 691-695

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Abstract

A comparative study was conducted to establish a diagnostic method for quickly and reliably detecting the Erysipelothrix genus during abattoir inspections. The study used three diagnostic methods: polymerase chainreaction (the PCR method), agglutination with antiserum to Erysipelothrix genus (the AG method) and routinepractice based on isolation and identification, in combination with cultivation in the enrichment broth (EB).The broth used was incubated for 20 hours under the PCR method, for 48 hours under the AG method and for20 hours (short-time method) and 48 hours (ordinary method) for routine practice. The ranking of the timetaken prior to a decision was: ordinary method>AG method>short-time method>PCR method. The examinationsfor detecting the Erysipelothrix genus in EB resulted in the following order of sensitivity: PCR method>routine practice>AG method. The steps between the minimum concentrations of bacteria for detectionwere about ten fold. The application of these methods to practical inspection of swine erysipelas in abattoirsresulted in the following order of accuracy: PCR method>ordinal method=AG method>short-timemethod, indicating that the PCR method combined with EB was a quick and reliable method for detecting Erysipelothrix genus.

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