Effect of Ethylene Thiourea on Cultured Rat Embryos in the Presence of Hepatic Microsomal Fraction

Takayuki IWASE; Masako YAMAMOTO; Mitsuyuki SHIRAI; Fumiaki AKAHORI; Toshio MASAOKA; Tatsuya TAKIZAWA; Kazuyoshi ARISHIMA; Yasunobu EGUCHI

doi:10.1292/jvms.59.59

Takayuki IWASE, Masako YAMAMOTO, Mitsuyuki SHIRAI, Fumiaki AKAHORI, Toshio MASAOKA, Tatsuya TAKIZAWA, Kazuyoshi ARISHIMA, Yasunobu EGUCHI

Author information

Takayuki IWASE
Department of Anatomy II, Azabu University, School of Veterinary Medicine
Masako YAMAMOTO
Department of Anatomy II, Azabu University, School of Veterinary Medicine
Mitsuyuki SHIRAI
Department of Veterinary Pharmacology, Azabi University, School of Veterinary Medicine
Fumiaki AKAHORI
Department of Veterinary Pharmacology, Azabi University, School of Veterinary Medicine
Toshio MASAOKA
Department of Developmental Biotechnology, Azabu University, School of Veterinary Medicine
Tatsuya TAKIZAWA
Department of Developmental Biotechnology, Azabu University, School of Veterinary Medicine
Kazuyoshi ARISHIMA
Department of Anatomy II, Azabu University, School of Veterinary Medicine
Yasunobu EGUCHI
Department of Anatomy II, Azabu University, School of Veterinary Medicine

Keywords: ethylene thiourea, hepatic microsomal fraction, rat embryo

JOURNAL FREE ACCESS

1997 Volume 59 Issue 1 Pages 59-61

DOI https://doi.org/10.1292/jvms.59.59

Details

Abstract

To investigate whether the teratogenicity of ethylene thiourea (ETU) is due to ETU per se or to its metabolites, rat embryos cultured in vitro were exposed to 10 and 30 μg/m l of ETU in the presence or absence of rat liver microsomal fraction (S9mix). In the absence of S9mix, the incidence of morphological abnormalities increased dose-dependently. In the presence of S9mix, abnormal morphogenesis was almost absent in all embryos. These findings suggest that teratogenicity of ETU is due to ETU per se.

Corresponding author

Register with J-STAGE for free!