Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
In Vitro Cytotoxicity of Recombinant Human-TNF-α and Actinomycin D on Canine Normal and Tumor Cells
Mica AOKIMitsuru KUWAMURATakao KOTANIHiromu KATAMOTOKihei KUBOKoichi NOMURANobuo SASAKIFumihito OHASHI
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1998 Volume 60 Issue 10 Pages 1087-1091

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Abstract

The in vitro cytotoxicity of recombinant human tumor necrosis factor-α (rh-TNF-α) and actinomycin D (ACT-D) on canine normal and tumor cells was investigated. rh-TNF-α showed dose-dependent cytotoxic and cell-growth inhibitory effects on cultured canine kidney carcinoma cells (CKCa-1). rh-TNF-α alone produced little cytotoxic effect on canine normal cells. However, combined with ACT-D, it showed moderate cytotoxicity on normal canine cells from the kidney medulla, spleen, heart muscle and lung. When the effects on the spontaneous tumor cells were examined, the combination of rh-TNF-α and ACT-D produced substantial cytotoxic effect on 60% of the tumor cells. All mammary mixed tumors and perianal gland tumors tested were susceptible to this combination. The data indicated the combination of rh-TNF-α and ACT-D have in vitro cytotoxicity on certain canine tumor cells.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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