Establishment of Boophilus microplus Infected with Babesia bigemina by Using in vitro Tube Feeding Technique

Abstract

The in vitro tube feeding technique is used to establish a laboratory colony of Boophilus microplus infected with Babesia bigemina. Pre-fed engorged female ticks offered 2 × 10⁴ and 2 × 10 ⁵/ml of B. bigemina infected bovine red blood cells (RBC) showed sporokinetes in the haemolymph smear sample, and positive signals for B. bigemina in polymerase chain reaction (PCR). Larvae laid from the ticks offered 2 × 10 ⁵/ml of B. bigemina infected RBC showed evidence for B. bigemina infection in microscopic method and PCR. While larvae laid from the ticks offered 2 × 10 ⁴/ml of B. bigemina infected RBC showed positive for B. bigemina in only PCR. The females offered 2 × 10³/ml B. bigemina infected RBC and their larvae did not show positive evidences for B. bigemina infection. It is thought that the in vitro tube feeding technique can be a convenient method to study the relationship between ticks and tick-borne pathogens. It is also suggested that the superior sensitivity of PCR compared to the microscopic method in detection of B. bigemina from the tick sample, especially in larvae.