Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Evaluation of Serological Diagnosis of Borna Disease Virus Infection Using Recombinant Proteins in Experimentally Infected Rats
Michiko OGINOKumiko YOSHIMATSUKouji TSUJIMURATetsuya MIZUTANIJiro ARIKAWAIkuo TAKASHIMA
Author information
Keywords: baculovirus, BDV, diagnosis
JOURNALS FREE ACCESS

1998 Volume 60 Issue 4 Pages 531-534

Details
Abstract

We produced two recombinant Borna disease virus (BDV) proteins, p40 and p24, by using a baculovirus vector as a diagnostic antigen. Antigenicities of these recombinant proteins were evaluated by immune rabbit sera. Recombinant p40 was a more sensitive antigen than p24 for the detection of antibodies in infected rats. Rats inoculated with BDV within 24 hr after birth showed higher detection rates of viral RNA and viral proteins from the brain than rats inoculated at 4 weeks-old. Depending on the age of infection and the time postinfection, the detection of BDV RNA, protein, or anti-BDV antibody did not always correlate in individuals. We suggest both serological and molecular biological methods are needed in the diagnosis of BDV infection.

Information related to the author

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
Previous article Next article
feedback
Top