Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
ELISA for Bovine Interleukin-1 Receptor Antagonist and Its Application to Mastitic Sera and Whey
Hitoki YAMANAKAKeiichi HISAEDAKatsuro HAGIWARARikio KIRISAWAHiroshi IWAI
著者情報
キーワード: bovine, ELISA, IL-1ra
ジャーナル フリー

2000 年 62 巻 6 号 p. 661-664

詳細
抄録

A sandwich ELISA for the bovine IL-1 receptor antagonist (bIL-1ra) was developed using recombinant (r) bIL-1ra produced by Escherichia coli, anti-rbIL-1ra rabbit IgG, its biotinylated one and avidin-peroxidase. This ELISA system enabled detection of rbIL-1ra at a concentration of more than 2 ng/ml. This ELISA was applied to quantitation of bIL-1ra in sera and whey of mastitic and healthy cows. The results indicate that although IL-1ra levels in healthy and mastitic sera and whey were comparable, serum IL-1ra/IL-1?b ratio of euthanized cows was significantly lower than that of the recovered.

著者関連情報

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
前の記事 次の記事
feedback
Top