Development of a Shuttle Polymerase Chain Reaction for the Detection of Bovine Herpesvirus 2
キーワード:
bovine herpesvirus 2 (BoHV-2),
PCR
2002 年 64 巻 10 号 p. 953-956
詳細
2002 年 64 巻 10 号 p. 953-956
Three different polymerase chain reaction (PCR) protocols were evaluated for their ability to detect bovine herpesvirus 2 (BoHV-2): single-step PCR with 3 reaction stages (denaturation, annealing and extension), 2 reaction stages (denaturation and annealing/extension; shuttle PCR), and semi-nested PCR with 3 reaction stages. All the PCR protocols showed the same sensitivity (detection limit of 0.4 TCID50). A non-specific band sometimes appeared in mock cell DNA at annealing temperatures below 64°C. The shuttle PCR was found to be superior to the other protocols under consideration because of the speed of its application. Furthermore, no non-specific band was detected in DNAs of eight other DNA viruses. Thus, the shuttle PCR seems to be an excellent diagnostic tool for BoHV-2 infections.
