Optimization of In situ Hybridization Protocols for Detection of Feline Herpesvirus 1
2003 年 65 巻 9 号 p. 1031-1032
詳細
2003 年 65 巻 9 号 p. 1031-1032
In situ hybridization (ISH) protocol including microwaving pre-treatment regimes was developed and compared with protease digestion as a pre-treatment regime for its effects on detecting feline herpesvirus 1 (FHV-1) in formalin fixed, paraffin embedded tissue. We found that optimum results were obtained using microwave pre-treatment. The results showed that the use of microwave irradiation would be recommended as a means of supplementing ISH methods, especially when using long-term formalin fixed, paraffin-embedded tissue.
