2004 Volume 66 Issue 12 Pages 1509-1515
We examined overlapping genomic clones containing the chicken T cell receptor (TCR) Dβ-Jβ-Cβ complex, which contains a single diversity segment, four joining segments and four exons that encode the constant region. This sequence comprised 18.3 kb. All four Jβ sequences possessed typical recombination signal sequences (RSS) with intervening 12-bp spacers at their 5'-ends and splice sites at their 3'-ends. No Jβ-pseudogenes were identified. TGTG sequences in the RSS heptamer sequences were well conserved, as is the case in mammals. A chicken repeat 1-like sequence was found in the intron region between Jβ-1336 and Cβ, and several small repeat sequences were identified in intron regions throughout this cloned genome. As germline sequences revealed complete Jβ sequences, the CDR3 (complementarity-determining region) sequences of TCRβ from non-immunized splenocytes were analyzed. Non-coding (N) and palindromic (P) nucleotides were frequently observed at the Dβ-Jβ recombination sites. There were differences in length of deletion at the 5'-end of each Jβ. Deletion of the 5'-end of Jβ-1280 was particularly short when compared with that of Jβ-1336, but there were no changes in the length of the CDR3 using any of the four Jβ sequences.