Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
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A Potential Live Vector, Foamy Virus, Directed Intra-Cellular Expression of Ovine Interferon-τ Exhibited the Resistance to HIV Infection
Yoichi FUJIIYasunori MURASEKaori OTAKEYasuko YOKOTAShinya OMOTOHidetoshi HAYASHIHidetika OKADANoriko OKADAMasahiro KAWAIHarumi OKUYAMAKazuhiko IMAKAWA
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2004 年 66 巻 2 号 p. 115-121

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Interferon-tau (IFN-τ), produced by the embryonic trophectoderm, is a member of type I IFNs required for the establishment of pregnancy in the ruminant ungulates. Although this IFN possesses antiviral activity similar to other type I IFNs, the effectiveness of IFN-τ as an antiviral agent has not been well characterized. To investigate possible antiviral effects of ovine IFN-τ (oIFN-τ), oIFN-τ-GST fusion protein was expressed in E. coli BL21, from which the purified protein isolated possessed anti-viral activity. An apathogenic human foamy virus (hFV) was then used to establish a potential recombinant live vector consisting of oIFN-τ cDNA sense (+) or antisense (-) sequence, oIFN-τ(+)/hFV or oIFN-τ(-)/hFV, respectively. Human hematopoietic and other mammalian cell lines that had been transduced with hFV vector consisting of no oIFN-τ, oIFN-τ(+)/hFV or oIFN-τ(-)/hFV construct were cultured initially for 12 days, and three of cell lines were then maintained for up to 90 days. These cells with oIFN-τ expression directed by hFV exhibited the in vitro cytopathic effect minimally. Transduced cell lines that had been cultured for 90 days were subjected to studies on human immunodeficiency virus type-1 (HIV-1) infection, which was measured with infectivity of viral particles resulted from the GFP inserted T-cell tropic HIV SF2 or macrophage tropic HIV SF162: the number of HIV-1 positive cells was reduced by the hFV driven-intra-cellular oIFN-τ expression. Since oIFN-τ/hFV transduced cells exhibited the resistance to HIV-1 infection and/or replication, oIFN-τ could be considered as one of effective antiviral agents against HIV-1. These results suggest that the hFV genome could be an effective recombinant live vector for the expression of a targeted gene in various cell types.

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© 2004 by the Japanese Society of Veterinary Science

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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