Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
FULL PAPER
Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene
Tadashi ITAGAKIMasayuki KIKAWAKunio TERASAKIToshiyuki SHIBAHARAKoichi FUKUDA
Author information
JOURNALS FREE ACCESS

2005 Volume 67 Issue 11 Pages 1115-1118

Details
Abstract

Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, Kor2a and Kor2b: F. gigantica-type). The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries.

Information related to the author
Previous article Next article
feedback
Top