An Immunohistochemical Study of Protein Kinase C in the Bovine Retina

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The expression of protein kinase C (PKC) was studied in the bovine retina by immunohistochemical analysis. Western blot analysis showed that PKC isoforms, including α, βI, δ and θ, were detected in the bovine retina. By immunohistochemistry, both PKC α and βI were expressed in all retinal layers, with an intense localization of both PKC α and βI detected in bipolar cells in the inner nuclear cell layer and in some glial cells in ganglion cell layers. The immunoreactivity of both PKC δ and θ was quite weak in the retinal layers, compared with that of PKC α and βI. These findings suggest that both conventional and novel PKCs are differentially expressed in the bovine retina.