Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
NOTE
High Level Expression of Biologically Active Canine Interferon-α Subtype 4 Using a Baculovirus
Ruttapong RUTTANAPUMMAManami ANZAIMasaoki TAKEGAWAMasashi OKAMURANobutoshi MAEHARAKo SATOKuniaki YOSHIOKAAkira ITOHMasayuki NAKAMURAKazuaki TAKEHARA
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2006 年 68 巻 12 号 p. 1347-1349

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In this study, a high amount of bioactive recombinant canine interferon-α subtype 4 (CaIFN-α4) was expressed in a baculovirus system. For easy purification, it was expressed as a CaIFN-α4 bearing histidine hexamer at the C-terminal region, designated CaIFN-α4His. CaIFN-α4His was detected in culture supernatants of insect cells infected with the recombinant virus using sodium dodecyl sulfate-polyarcylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue staining. The level of expression was very high, and approximately 1 mg of purified protein, with 5.0 × 107 units/mg, was obtained from 300 ml of culture supernatant. The purified product showed antiviral activity against Vesicular stomatitis virus on canine tumor cell line A72 and chicken embryo fibroblast cells.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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