A goat with neurologic signs had multifocal abscesses containing sulfur granules in the right brain and temporal bone. Histologically, the lesions consisted of pyogranulomas with several radiating bacterial colonies of various sizes. A tangled mass of filamentous and gram-positive bacteria was recognized in the central part of the colony. Actinomyces naeslundii antigen was detected in the colonies of bacteria in the brain and neighboring bone tissue by immunohistochemistry. Actinomycosis involving the central nervous system (CNS) and temporal bone is rare in animals. Cerebral infection with A. naeslundii may have resulted from direct extension from cervicofacial regions because the CNS lesions were distributed asymmetrically and were continuous with the right temporal bone.