FULL PAPER
Cyclosporine A Inhibits the mRNA Expressions of IL-2, IL-4 and IFN-γ, but not TNF-α, in Canine Mononuclear Cells
2007 年 69 巻 9 号 p. 887-892
詳細
2007 年 69 巻 9 号 p. 887-892
The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-γ and TNF-α were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and IFN-γ mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-α. These findings are important for assessing the indications of CsA treatment in dogs.
