Molecular Detection of Mycoplasma haemofelis and `Candidatus Mycoplasma haemominutum' Infection in Cats by Direct PCR Using Whole Blood without DNA Extraction

Masashi WATANABE; Masaharu HISASUE; Takehisa SOUMA; Shuichi OHSHIRO; Takatsugu YAMADA; Ryo TSUCHIYA

doi:10.1292/jvms.70.1095

Clinical Pathology

Molecular Detection of Mycoplasma haemofelis and `Candidatus Mycoplasma haemominutum' Infection in Cats by Direct PCR Using Whole Blood without DNA Extraction

Masashi WATANABE, Masaharu HISASUE, Takehisa SOUMA, Shuichi OHSHIRO, Takatsugu YAMADA, Ryo TSUCHIYA

著者情報

キーワード: blood, feline, hemoplasma

ジャーナルフリー

2008 年 70 巻 10 号 p. 1095-1099

DOI https://doi.org/10.1292/jvms.70.1095

詳細

抄録

Detection of hemotropic Mycoplasma spp. infection was attempted in cats by PCR using whole blood without DNA extraction. A total 46 of 54 (85%) cats with suspected Mycoplasma spp. infection showed a positive reaction, corresponding completely with the results of standard PCR testing. The direct PCR assay was sensitive enough to detect more than 0.0061% parasitemia for `C. M. haemominutum' and 0.0075% parasitemia for M. haemofelis. These data indicate that the direct PCR assay might be sufficient for use as a tool in clinical examinations.

責任著者(Corresponding author)

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