Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Clinical Pathology
26S rDNA-Based Phylogenetic Investigation of Japanese Cattle-Associated Prototheca zopfii Isolates
Yuji KISHIMOTORui KANOHaruhiko MARUYAMAMasanobu ONOZAKIKoichi MAKIMURATakaaki ITOKiyoshi MATSUBARAAtsuhiko HASEGAWAHiroshi KAMATA
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2010 年 72 巻 1 号 p. 123-126

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The study has been conducted to assess the applicability of partial 26S ribosomal DNA sequence for the phylogenic analysis of P.zopfii and to arrive relationships between specific genotype of P.zopfii and clinical mastitis. The phylogenetic analysis indicated that all genotype 2 isolates were grouped into the cluster of type strain of P.zopfii. Thus, all mastitis isolates in Japan were identified as P.zopfii genotype 2 and were independent of the cluster of the genotype 1 isolates. In one clinical case, a fecal isolate could not be identified by the 18S rDNA-based genotype specific PCR-assay and appeared to belong to a same cluster with the type strain of P. blaschkeae by 26S rDNA analysis. The isolate was the first isolation in Japan.

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© 2010 by the Japanese Society of Veterinary Science

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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