Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
Internal Medicine
Application of Gel Permeation HPLC for Lipoprotein Profiling in Dogs
Hisashi MIZUTANIToshinori SAKONoriaki ARAIKoji KURIYAMAItaru YOSHIMURAAkihiro MORIKenji IWASEHisashi HIROSE
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2010 Volume 72 Issue 6 Pages 813-817

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Abstract

A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28-29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.

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