Antimicrobial Susceptibility and PFGE Genotyping of Haemophilus parasuis Isolates from Pigs in South China (2008-2010)

Chenggang XU; Jianmin ZHANG; Zhanqin ZHAO; Lili GUO; Bin ZHANG; Saixiang FENG; Lingyun ZHANG; Ming LIAO

doi:10.1292/jvms.10-0515

Bacteriology

Antimicrobial Susceptibility and PFGE Genotyping of Haemophilus parasuis Isolates from Pigs in South China (2008-2010)

Chenggang XU, Jianmin ZHANG, Zhanqin ZHAO, Lili GUO, Bin ZHANG, Saixiang FENG, Lingyun ZHANG, Ming LIAO

Author information

Chenggang XU
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University
Jianmin ZHANG
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University
Zhanqin ZHAO
Henan University of Science and Technology
Lili GUO
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University
Bin ZHANG
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University
Saixiang FENG
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University
Lingyun ZHANG
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University
Ming LIAO
Key Laboratory of Animal Disease Control and Prevention of the Ministry of Agriculture, Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University

Keywords: antimicrobial susceptibility, Haemophilus parasuis, PFGE, swine

JOURNAL FREE ACCESS

2011 Volume 73 Issue 8 Pages 1061-1065

DOI https://doi.org/10.1292/jvms.10-0515

View "Advance Publication" version (April 4, 2011).

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Abstract

H. parasuis isolates (n=112) from pigs were tested for antimicrobial susceptibility against 23 antimicrobial agents by the disk diffusion method. All isolates were sensitive to Florfenico and most strains were sensitive to Cefotaxime (103/112; 92%), Ceftazidime (99/112; 88.4%), Chloramphenicol (90/112; 80.4%) and Gentamicin (85/112; 75.9%). High resistance levels to Nalidixic acid (84.8%), TMP (67.9%) and Trimethoprim+Sulfamethoxazole (58%) were observed. Genomic DNA extracted from 52 isolates resistant to at least seven antimicrobial agents was analyzed by PFGE and 46 distinct PFGE patterns identified. Diverse variation was observed between the drug-resistant H. parasuis isolates examined, suggesting that resistance traits were acquired independently by the respective isolates.

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