Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Virology
Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing
Fujiko MINAMI-FUKUDAMakoto NAGAIHikaru TAKAIToshiaki MURAKAMITadashi OZAWAShinobu TSUCHIAKASachiko OKAZAKIYukie KATAYAMAMami OBANaomi NISHIURAYukiko SASSATsutomu OMATSUTetsuya FURUYASatoshi KOYAMAJunsuke SHIRAIHiroshi TSUNEMITSUYoshiki FUJIIKazuhiko KATAYAMATetsuya MIZUTANI
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2013 Volume 75 Issue 12 Pages 1651-1655

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Abstract

We investigated the sensitivity of human rotavirus rapid antigen detection (RAD) kits, RT-PCR and next-generation DNA sequencing (NGS) for detection of bovine group A rotavirus (RVA). The Dipstick ‘Eiken’ Rota (Dipstick) showed the highest sensitivity out of the seven RAD kits against all selected strains in limited dilution analyses, which was consistent with the results for equine rotavirus previously reported. RT-PCR had 100–103-fold higher sensitivity than the Dipstick. NGS using thirteen RT-PCR-negative fecal samples revealed that all samples yielded RVA reads and especially that two of them covered all 11 genome segments. Moreover, mapping reads to reference sequences allowed genotyping. The NGS would be sensitive and useful for analysis of less dependent on specific primers and screening of genotypes.

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この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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