Improvement of Preimplantation Development of In          Vitro-Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically          Defined Medium

Nobutada SAKAGAMI; Osamu NISHINO; Satoshi ADACHI; Hidenobu UMEKI; Hiroko UCHIYAMA; Kyoko ICHIKAWA; Kazuhisa TAKESHITA; Etsushi KANEKO; Kiyoshi AKIYAMA; Shuji KOBAYASHI; Hiromichi TAMADA

doi:10.1292/jvms.13-0543

Theriogenology

Improvement of Preimplantation Development of In Vitro-Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium

Nobutada SAKAGAMI, Osamu NISHINO, Satoshi ADACHI, Hidenobu UMEKI, Hiroko UCHIYAMA, Kyoko ICHIKAWA, Kazuhisa TAKESHITA, Etsushi KANEKO, Kiyoshi AKIYAMA, Shuji KOBAYASHI, Hiromichi TAMADA

Author information

Nobutada SAKAGAMI
Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243–0417, Japan
Osamu NISHINO
Nara Prefectural Livestock Technology Center, Nara 633–1302, Japan
Satoshi ADACHI
Ooita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Ooita 878–0201, Japan
Hidenobu UMEKI
Ooita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Ooita 878–0201, Japan
Hiroko UCHIYAMA
Miyazaki Prefectural Livestock Research Institute, Miyazaki 889–4411, Japan
Kyoko ICHIKAWA
Kochi Prefectural Livestock Research Institute, Kochi 789–1233, Japan
Kazuhisa TAKESHITA
Yamaguchi Prefectural Livestock Experiment Station, Yamaguchi 759–2221, Japan
Etsushi KANEKO
Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243–0417, Japan
Kiyoshi AKIYAMA
Kanagawa Prefectural Livestock Industry Technology Center, Ebina, Kanagawa 243–0417, Japan
Shuji KOBAYASHI
National Livestock Breeding Center, Fukushima 961–8511, Japan
Hiromichi TAMADA
Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 598–8531, Japan

Keywords: bovine, chemically defined medium, embryo, embryo culture, glucose

JOURNAL FREE ACCESS

2014 Volume 76 Issue 10 Pages 1403-1405

DOI https://doi.org/10.1292/jvms.13-0543

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Abstract

The influences of glucose supplementation on early development of bovine embryos in BSA-free synthetic oviduct fluid were examined. Among the groups supplemented with 1.5, 2.0, 4.0 or 5.6 mM glucose either at 0, 72 or 144 hr after fertilization, blastocysts yield significantly increased in the group supplemented with 4.0 mM glucose 144 hr after fertilization compared to the controls without glucose supplementation. The results suggest that appropriate amounts of glucose supplemented to the medium at the specific stage of embryo culture may be useful for the production of bovine blastocysts.

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