Nutrient starvation affects expression of LC3 family at the feto-maternal          interface during murine placentation

Masato HIYAMA; Ken Takeshi KUSAKABE; Ai TAKESHITA; Sinji SUGI; Nobue KUNIYOSHI; Hiroyuki IMAI; Kiyoshi KANO; Yasuo KISO

doi:10.1292/jvms.14-0490

Anatomy

Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation

Masato HIYAMA, Ken Takeshi KUSAKABE, Ai TAKESHITA, Sinji SUGI, Nobue KUNIYOSHI, Hiroyuki IMAI, Kiyoshi KANO, Yasuo KISO

著者情報

Masato HIYAMA
Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan
Ken Takeshi KUSAKABE
Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan
Ai TAKESHITA
Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University, Osaka 598–8531, Japan
Sinji SUGI
Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan
Nobue KUNIYOSHI
Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan
Hiroyuki IMAI
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan
Kiyoshi KANO
Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan
Yasuo KISO
Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan

キーワード: autophagy, LC3, mTOR, placenta, starvation

ジャーナルフリー

2015 年 77 巻 3 号 p. 305-311

DOI https://doi.org/10.1292/jvms.14-0490

Browse “Advance online publication” version

詳細

抄録

LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.

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