Molecular typing of Salmonella enterica serovar 4,[5],12:i:-          isolates from humans, animals and river water in Japan by multilocus variable-number          tandem repeat analysis and pulsed-field gel electrophoresis

Noriko IDO; Kaori IWABUCHI; Yusuke SATO’O; Yasuo SATO; Masaru SUGAWARA; Gakuji YAEGASHI; Masaru KONNO; Masato AKIBA; Kiyoshi TANAKA; Katsuhiko OMOE; Ikuo UCHIDA

doi:10.1292/jvms.14-0465

Public Health

Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis

Noriko IDO, Kaori IWABUCHI, Yusuke SATO’O, Yasuo SATO, Masaru SUGAWARA, Gakuji YAEGASHI, Masaru KONNO, Masato AKIBA, Kiyoshi TANAKA, Katsuhiko OMOE, Ikuo UCHIDA

著者情報

Noriko IDO
Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan
Kaori IWABUCHI
Research Institute for Environmental Sciences and Public Health of Iwate Prefecture, Morioka, Japan
Yusuke SATO’O
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan
The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan
Yasuo SATO
Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan
Masaru SUGAWARA
Iwate Prefecture North Livestock Hygiene Service Center, Karumai, Japan
Gakuji YAEGASHI
Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa, Japan
Masaru KONNO
Iwate Agricultural Research Center, Taneyama livestock laboratory, Oshu, Japan
Masato AKIBA
National Institute of Animal Health, Tsukuba, Japan
Kiyoshi TANAKA
Hokkaido Research Station, National Institute of Animal Health, Sapporo, Japan
Katsuhiko OMOE
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka, Japan
The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan
Ikuo UCHIDA
The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan
Hokkaido Research Station, National Institute of Animal Health, Sapporo, Japan

キーワード: MLVA, monophasic variant, PFGE, Salmonella 4,[5],12:i:-(S. 4, [5],12:i:-), S. Typhimurium

ジャーナルフリー

2015 年 77 巻 5 号 p. 609-613

DOI https://doi.org/10.1292/jvms.14-0465

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詳細

抄録

Fifty-one Salmonella enterica serovar 4,[5],12:i:- (S. 4, [5],12:i:-) isolates (14 human strains, 34 animal strains and 3 river water strains) which are assumed to be monophasic variants of S. Typhimurium were analyzed using pulsed-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA) in order to investigate their genetic diversities and relationships. PFGE, MLVA and combination of them identified 28, 27 and 34 profiles (Simpson’s diversity indices [DI]=0.94, 0.96 and 0.97), respectively. No correlations were detected between MLVA clustering and PFGE clustering or phage typing. These results suggested that S. 4,[5],12:i:- originated from multiple S. Typhimurium ancestors. Two cattle and one pig isolates showing identical phage types as well as PFGE and MLVA profiles to human isolates S. 4,[5],12:i:- suggested the existence of the links between human infections and animal reservoirs.

責任著者(Corresponding author)

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