The cell wall component lipoteichoic acid of Staphylococcus            aureus induces chemokine gene expression in bovine mammary epithelial          cells

Yoshio KIKU; Yuya NAGASAWA; Fuyuko TANABE; Kazue SUGAWARA; Atsushi WATANABE; Eiji HATA; Tomomi OZAWA; Kei-ichi NAKAJIMA; Toshiro ARAI; Tomohito HAYASHI

doi:10.1292/jvms.15-0706

Immunology

The cell wall component lipoteichoic acid of Staphylococcus aureus induces chemokine gene expression in bovine mammary epithelial cells

Yoshio KIKU, Yuya NAGASAWA, Fuyuko TANABE, Kazue SUGAWARA, Atsushi WATANABE, Eiji HATA, Tomomi OZAWA, Kei-ichi NAKAJIMA, Toshiro ARAI, Tomohito HAYASHI

Author information

Yoshio KIKU
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan
Yuya NAGASAWA
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan
Fuyuko TANABE
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan
Kazue SUGAWARA
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan
Atsushi WATANABE
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan
Eiji HATA
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan
Tomomi OZAWA
National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305–0856, Japan
Kei-ichi NAKAJIMA
Hokkaido Agricultural Research Center, NARO, Sapporo, Hokkaido 062–8555, Japan
Toshiro ARAI
School of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo 180–8602, Japan
Tomohito HAYASHI
Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido 062–0045, Japan

Keywords: bovine mammary epithelial cell, cap analysis of gene expression, chemokine, lipoteichoic acid, Staphylococcus aureus

JOURNAL FREE ACCESS

2016 Volume 78 Issue 9 Pages 1505-1510

DOI https://doi.org/10.1292/jvms.15-0706

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Abstract

Staphylococcus aureus (SA) is a major cause of bovine mastitis, but its pathogenic mechanism remains poorly understood. To evaluate the role of lipoteichoic acid (LTA) in the immune or inflammatory response of SA mastitis, we investigated the gene expression profile in bovine mammary epithelial cells stimulated with LTA alone or with formalin-killed SA (FKSA) using cap analysis of gene expression. Seven common differentially expressed genes related to immune or inflammatory mediators were up-regulated under both LTA and FKSA stimulations. Three of these genes encode chemokines (IL-8, CXCL6 and CCL2) functioning as chemoattractant molecules for neutrophils and macrophages. These results suggest that the initial inflammatory response of SA infection in mammary gland may be related with LTA induced chemokine genes.

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