Single nucleotide polymorphism genotyping of Erysipelothrix            rhusiopathiae isolates from pigs affected with chronic erysipelas in          Japan

Kazumasa SHIRAIWA; Yohsuke OGAWA; Sayaka NISHIKAWA; Masahiro KUSUMOTO; Masahiro EGUCHI; Yoshihiro SHIMOJI

doi:10.1292/jvms.17-0040

Bacteriology

Single nucleotide polymorphism genotyping of Erysipelothrix rhusiopathiae isolates from pigs affected with chronic erysipelas in Japan

Kazumasa SHIRAIWA, Yohsuke OGAWA, Sayaka NISHIKAWA, Masahiro KUSUMOTO, Masahiro EGUCHI, Yoshihiro SHIMOJI

著者情報

Kazumasa SHIRAIWA
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Yohsuke OGAWA
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Sayaka NISHIKAWA
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Masahiro KUSUMOTO
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Masahiro EGUCHI
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Yoshihiro SHIMOJI
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
Research Institute for Biomedical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan

キーワード: Erysipelothrix rhusiopathiae, Koganei 65-0.15 strain, SNP-based PCR, strain-specific detection, vaccine

ジャーナルフリー

2017 年 79 巻 4 号 p. 699-701

DOI https://doi.org/10.1292/jvms.17-0040

Browse “Advance online publication” version

詳細

抄録

Over the past decades, Erysipelothrix rhusiopathiae strains displaying similar phenotypic and genetic profiles of the attenuated, acriflavine-resistant E. rhusiopathiae Koganei 65-0.15 strain (serovar 1a) have been frequently isolated from pigs affected with chronic erysipelas in Japan. In this study, using the conventional PCR assay that was designed to detect strain-specific single nucleotide polymorphism (SNP) sites found in the genome of the vaccine strain, we analyzed E. rhusiopathiae isolates from pigs with chronic disease in farms where the Koganei vaccine was used. Out of a total of 155 isolates, 101 isolates (65.2%) were determined to be the vaccine strain by SNP-based PCR. Among the 101 PCR-positive isolates, four isolates were found to be sensitive to acriflavine.

責任著者(Corresponding author)

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